Changing human behaviours can deliver considerable conservation benefits but which behavioural changes are likely to deliver the greatest benefits for biodiversity? We applied a strategic prioritisation approach, with an expert elicitation workshop and follow-up surveys to identify the top 10 behaviours that the typical Victorian citizen can participate in that will have the most impact for biodiversity conversation outcomes. We considered the level of biodiversity benefit, the capacity for change and the existing prevalence of the action. Although this work was focused on Victoria, the priority behaviours that we identified for biodiversity outcomes are likely representative for much of Australia.